Define where the pipeline should find input data and save output data.

Path to comma-separated or tab-separated file containing information about the samples in the experiment.

required
type: string
pattern: ^\S+\.(csv|tsv)$

The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.

required
type: string

Email address for completion summary.

type: string
pattern: ^([a-zA-Z0-9_\-\.]+)@([a-zA-Z0-9_\-\.]+)\.([a-zA-Z]{2,5})$

Parameters used to describe centralised config profiles. These should not be edited.

Git commit id for Institutional configs.

hidden
type: string
default: master

Base directory for Institutional configs.

hidden
type: string
default: https://raw.githubusercontent.com/nf-core/configs/master

Institutional config name.

hidden
type: string

Institutional config description.

hidden
type: string

Institutional config contact information.

hidden
type: string

Institutional config URL link.

hidden
type: string

Less common options for the pipeline, typically set in a config file.

Display version and exit.

hidden
type: boolean

Method used to save pipeline results to output directory.

hidden
type: string

Email address for completion summary, only when pipeline fails.

hidden
type: string
pattern: ^([a-zA-Z0-9_\-\.]+)@([a-zA-Z0-9_\-\.]+)\.([a-zA-Z]{2,5})$

Send plain-text email instead of HTML.

hidden
type: boolean

Do not use coloured log outputs.

hidden
type: boolean

Boolean whether to validate parameters against the schema at runtime

hidden
type: boolean
default: true

Base URL or local path to location of pipeline test dataset files

hidden
type: string
default: https://raw.githubusercontent.com/nf-core/test-datasets/

Suffix to add to the trace report filename. Default is the date and time in the format yyyy-MM-dd_HH-mm-ss.

hidden
type: string

Display the help message.

type: boolean,string

Display the full detailed help message.

type: boolean

Display hidden parameters in the help message (only works when –help or –help_full are provided).

type: boolean

Options for genome annotation

Tool to use for genome annotation

type: string

Directory containing a pre-downloaded Bakta database, or one published by a previous run of this pipeline (only used when annotation_tool=‘bakta’). If unset, the database is downloaded automatically into <outdir>/databases/bakta on first use and reused from there by later runs that point --bakta_db at the same <outdir>/databases/bakta.

type: string

Bakta database type to download

type: string

Minimum contig length for annotation and analysis

type: integer
default: 200

Options for required databases

Directory containing a pre-downloaded Pfam database, or one published by a previous run of this pipeline (only used when skip_traitar=false). If unset, the database is downloaded automatically into <outdir>/databases/pfam on first use and reused from there by later runs that point --pfamdb at the same <outdir>/databases/pfam.

type: string

Options for functional annotation and metabolic modeling tools

Skip MacSyFinder macromolecular systems detection

type: boolean

MacSyFinder models to use (TXSS for Type III/IV/VI secretion systems)

type: string
default: TXSS

MacSyFinder –db-type: unordered for MAGs/draft assemblies with unreliable gene order (default, most permissive), ordered_replicon for a single complete/nearly-complete genome (enables synteny-aware detection), or gembase for multiple complete replicons in one gembase-formatted database

type: string

Directory containing pre-downloaded MacSyFinder models, or ones published by a previous run of this pipeline (one subdirectory per macsyfinder_models value). If unset, models are downloaded automatically into <outdir>/databases/macsyfinder on first use and reused from there by later runs that point --macsyfinder_db at the same <outdir>/databases/macsyfinder.

type: string

Skip TRAITAR phenotype prediction

type: boolean

Skip CarveMe genome-scale metabolic reconstruction

type: boolean

Path to CarveMe media database TSV file (columns: medium, description, compound, name). If not provided, uses CarveMe’s default media database. See example file.

type: string

Skip Gapseq pathway analysis and metabolic modeling

type: boolean
default: true

Directory containing a pre-downloaded gapseq reference sequence database (a ‘Bacteria’ subdirectory, as produced by gapseq update-sequences -D <dir>). If present, the live Zenodo download is skipped - set this to a pre-downloaded copy if your compute nodes lack outbound internet access to Zenodo. If unset, defaults to <outdir>/databases/gapseq.

type: string

Custom arguments for the simplified gapseq doall search step. Only used in the default doall workflow - ignored if any of the gapseq_find_args, gapseq_findtransport_args, gapseq_draft_args, gapseq_medium_args, or gapseq_fill_args below are set, since that switches to the custom/stepwise workflow instead.

type: string
default: -b 200 -A diamond

Custom arguments for ‘gapseq find’ - pathway prediction (default: ‘-b 200 -A diamond’)

type: string

Custom arguments for ‘gapseq find-transport’ - transporter prediction (default: ‘-b 200’)

type: string

Custom arguments for ‘gapseq draft’ - draft model construction (default: none)

type: string

Custom arguments for ‘gapseq medium’ - add/remove specific compounds on top of the predicted medium, e.g. ‘-c “cpd00007:0”’ to remove oxygen (default: none; ignored for samples with their own medium_gapseq_csv file)

type: string

Custom arguments for ‘gapseq fill’ - gap-filling (default: none)

type: string

Skip MEMOTE metabolic model quality evaluation (only runs when skip_gapseq=false or skip_carveme=false)

type: boolean
default: true